A Complement Fixation Test for Poliomyelitis

The use of new antigens in the complementfixation test for acute poliomyelitis.

Hitherto in poliomyelitis complement-fixation tests on human sera the antigenic material has usually been derived from tissue cultures of polio viruses. Despite high infectivity titres, antigens from this source are weak in capacity to fix complement (Selzer, 1958). Concentration of tissue culture antigens improves the complement-fixing power but frequently produces anti-complementary effects. ...

Complement fixation test using the antigen prepared from the nervous tissue of adult mice infected with poliomyelitis virus Lansing type.

The technique of complement fixation test as a measure for the serological diagnosis of neurotropic virus infections was first put in practice by Casals and Palacios (1) in the year 1941, and confirmed of its practical diagnostic value by numerous follow-ups made after it. In spite of repeated attempts, the application of this technique for the diagnosis of poliomyelitis had not been successful...

Complement Fixation Tests in Poliomyelitis **

Infection with poliomyelitis virus gives rise to complement-fixing (C-F) antibodies which may be detected with antigens prepared either from the brains of infected mice or tissue culture fluids, as shown with Type II by Casals et al."' and with all three types by Svedmyr et al.' These workers used sufficient antigen in each test to obtain maximum sensitivity. With this antigen concentration the...

A Specific Complement-fixation Test for Infection with Poliomyelitis Virus

A complement-fixing antigen has been developed, using as source of material CNS tissue from newborn mice infected with the newborn mouse-adapted strain of the Lansing type, MEF1 virus. With this antigen, specific reactions have been obtained with sera from mice, cotton rats, and monkeys immunized with the Lansing-type virus, and from monkeys and chimpanzees convalescent from infection with this...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test